Evaluation of weak interactions of proteins and organic cations with DNA duplex structures.

ABSTRACT: Molecular interactions and reactions in living cells occur with high background concentrations of organic compounds including proteins. Uncharged water-soluble polymers are commonly used cosolutes in studies on molecular crowding, and most studies argue about the effects of intracellular crowding based on results obtained using polymer cosolutes. Further investigations using protein crowders and organic cations are important in understanding the effects of cellular environments on nucleic acids with negatively charged surfaces. We assessed the effects of using model globular proteins, serum proteins, histone proteins, structurally flexible polypeptides, di- and polyamines, and uncharged polymers. Thermal stability analysis of DNA oligonucleotide structures revealed that unlike conventional

SUBMITTER: Morimoto R 

PROVIDER: S-EPMC9388550 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature