Single-cell Transcriptomics Reveals Dynamic Role of Smooth Muscle Cells and Enrichment of Immune Cell Subsets in Human Abdominal Aortic Aneurysms.

ABSTRACT:

Objective

To determine cell-specific gene expression profiles that contribute to development of abdominal aortic aneurysms (AAAs).

Background

AAAs represent the most common pathological aortic dilation leading to the fatal consequence of aortic rupture. Both immune and structural cells contribute to aortic degeneration, however, gene specific alterations in these cellular subsets are poorly understood.

Methods

We performed single-cell RNA sequencing (scRNA-seq) analysis of AAAs and control tissues. AAA-related changes were examined by comparing gene expression profiles as well as detailed receptor-ligand interactions. An integrative analysis of scRNA-seq data with large genome-wide association study data was conducted to identify genes critical for AAA development.

SUBMITTER: Davis FM 

PROVIDER: S-EPMC9388616 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature