Hypoxia-ischemia-mediated effects on neurodevelopmentally regulated cold-shock proteins in neonatal mice under strict temperature control.

ABSTRACT:

Background

Neonates have high levels of cold-shock proteins (CSPs) in the normothermic brain for a limited period following birth. Hypoxic-ischemic (HI) insults in term infants produce neonatal encephalopathy (NE), and it remains unclear whether HI-induced pathology alters baseline CSP expression in the normothermic brain.

Methods

Here we established a version of the Rice-Vannucci model in PND 10 mice that incorporates rigorous temperature control.

Results

Common carotid artery (CCA)-ligation plus 25 min hypoxia (8% O₂) in pups with targeted normothermia resulted in classic histopathological changes including increased hippocampal degeneration, astrogliosis, microgliosis, white matter changes, and cell signaling perturbations. Serial assessment of cortical,

SUBMITTER: Jackson TC 

PROVIDER: S-EPMC9388702 | biostudies-literature | 2022 Feb

REPOSITORIES: biostudies-literature