An optimized protocol for assessment of sputum macrorheology in health and muco-obstructive lung disease.

ABSTRACT: Background: Airway mucus provides important protective functions in health and abnormal viscoelasticity is a hallmark of muco-obstructive lung diseases such as cystic fibrosis (CF). However, previous studies of sputum macrorheology from healthy individuals and patients with CF using different experimental protocols yielded in part discrepant results and data on a systematic assessment across measurement settings and conditions remain limited. Objectives: The aim of this study was to develop an optimized and reliable protocol for standardized macrorheological measurements of airway mucus model systems and native human sputum from healthy individuals and patients with muco-obstructive lung disease. Methods: Oscillatory rheological shear measurements were performed using

SUBMITTER: Voller M 

PROVIDER: S-EPMC9388721 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature