Identification and characterization of N6-methyladenosine circular RNAs in the spinal cord of morphine-tolerant rats.

ABSTRACT: Morphine tolerance (MT) is a tricky problem, the mechanism of it is currently unknown. Circular RNAs (circRNAs) serve significant functions in the biological processes (BPs) of the central nervous system. N6-methyladenosine (m⁶A), as a key post-transcriptional modification of RNA, can regulate the metabolism and functions of circRNAs. Here we explore the patterns of m⁶A-methylation of circRNAs in the spinal cord of morphine-tolerant rats. In brief, we constructed a morphine-tolerant rat model, performed m⁶A epitranscriptomic microarray using RNA samples collected from the spinal cords of morphine-tolerant rats and normal saline rats, and implemented the bioinformatics analysis. In the spinal cord of morphine-tolerant rats, 120 circRNAs with different m

SUBMITTER: Xing M 

PROVIDER: S-EPMC9388936 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature