Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria.
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ABSTRACT:
SUBMITTER: Li C
PROVIDER: S-EPMC9389416 | biostudies-literature | 2022 Sep
REPOSITORIES: biostudies-literature
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Protocol for using autoclaved intertidal sediment as a medium to enrich marine cable bacteria.
Li Cheng C Reimers Clare E CE Chace Peter J PJ
STAR protocols 20220811 3
Cable bacteria (CB) are non-isolated filamentous bacteria in the family of <i>Desulfobulbaceae</i>, known for fostering centimeter-long electron transfer in sediments with pronounced redox zonation. This protocol details steps to extract CB filaments from cultured natural sediment, inoculate autoclaved sediment with extracted filaments, and subsequently evaluate the growth and enrichment of CB. We also describe the approaches for collecting suitable sediment, preparing autoclaved sediment, and m ...[more]
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