An affinity for brainstem microglia in pediatric high-grade gliomas of brainstem origin.

ABSTRACT:

Background

High-grade gliomas (HGG) in children have a devastating prognosis and occur in a remarkable spatiotemporal pattern. Diffuse midline gliomas (DMG), including diffuse intrinsic pontine gliomas (DIPG), typically occur in mid-childhood, while cortical HGGs are more frequent in older children and adults. The mechanisms behind this pattern are not clear.

Methods

We used mouse organotypic slice cultures and glial cell cultures to test the impact of the microenvironment on human DIPG cells. Comparing the expression between brainstem and cortical microglia identified differentially expressed secreted proteins. The impact of some of these proteins on DIPGs was tested.

Results

DIPGs, pediatric HGGs of brainstem origin, survive and divide more in organotypic slice cult

SUBMITTER: Zats LP 

PROVIDER: S-EPMC9389428 | biostudies-literature | 2022 Jan-Dec

REPOSITORIES: biostudies-literature