Copper-Catalyzed Glutathione Oxidation is Accelerated by the Anticancer Thiosemicarbazone Dp44mT and Further Boosted at Lower pH.

ABSTRACT: Glutathione (GSH) is the most abundant thiol in mammalian cells and plays a crucial role in maintaining redox cellular homeostasis. The thiols of two GSH molecules can be oxidized to the disulfide GSSG. The cytosolic GSH/GSSG ratio is very high (>100), and its reduction can lead to apoptosis or necrosis, which are of interest in cancer research. Cu^II ions are very efficient oxidants of thiols, but with an excess of GSH, Cu^I_n(GS)_m clusters are formed, in which Cu^I is very slowly reoxidized by O₂ at pH 7.4 and even more slowly at lower pH. Here, the aerobic oxidation of GSH by Cu^II was investigated at different pH values in the presence of the anticancer thiosemicarbazone Dp44mT, which accumulates in l

SUBMITTER: Falcone E 

PROVIDER: S-EPMC9389589 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature