CDC50A might be a novel biomarker of epithelial ovarian cancer-initiating cells.

ABSTRACT:

Background

The aim of this work was to screen and validate biomarkers of ovarian cancer-initiating cells to detect the mechanisms of recurrence of epithelial ovarian cancer (EOC).

Methods

Stably labelled the amino acid in side population (SP) cells of epithelial ovarian cancer which were rich in cancer-initiating cells and non-SP cells with isotope in culture and differentially expressed cellular membrane proteins in SP cells were identified through proteomics technology. The new candidate biomarker was screened and validated through RT-PCR and western blot. Both in cell lines and primary EOC, cancer-initiating biofunctions of CDC50A positive cells were validated. Moreover, the characteristics of mesenchymal transition (EMT) was also detected and the correlation between the

SUBMITTER: Yin J 

PROVIDER: S-EPMC9389740 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature