Biochemical Characterization of Emerging SARS-CoV-2 Nsp15 Endoribonuclease Variants.

ABSTRACT: Global sequencing efforts from the ongoing COVID-19 pandemic, caused by the novel coronavirus SARS-CoV-2, continue to provide insight into the evolution of the viral genome. Coronaviruses encode 16 nonstructural proteins, within the first two-thirds of their genome, that facilitate viral replication and transcription as well as evasion of the host immune response. However, many of these viral proteins remain understudied. Nsp15 is a uridine-specific endoribonuclease conserved across all coronaviruses. The nuclease activity of Nsp15 helps the virus evade triggering an innate immune response. Understanding how Nsp15 has changed over the course of the pandemic, and how mutations affect its RNA processing function, will provide insight into the evolution of an oligomerization-dependent endorib

SUBMITTER: Wilson IM 

PROVIDER: S-EPMC9389836 | biostudies-literature | 2022 Oct

REPOSITORIES: biostudies-literature