NMR metabolomic analysis of bacterial resistance pathways using multivalent quaternary ammonium functionalized macromolecules.

ABSTRACT:

Introduction

Multivalent antimicrobial dendrimers are an exciting new system that is being developed to address the growing problem of drug resistant bacteria. Nuclear Magnetic Resonance (NMR) metabolomics is a quantitative and reproducible method for the determination of bacterial response to environmental stressors and for visualization of perturbations to biochemical pathways.

Objectives

NMR metabolomics is used to elucidate metabolite differences between wild type and antimicrobially mutated Escherichia coli (E. coli) samples.

Methods

Proton (¹H) NMR hydrophilic metabolite analysis was conducted on samples of E. coli after 33 growth cycles of a minimum inhibitory challenge to E. coli by poly(amidoamine) dendrimers functionalized with mannose and with C<

SUBMITTER: Aries ML 

PROVIDER: S-EPMC9389846 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature