Therapeutic potential of clinical-grade human induced pluripotent stem cell-derived cardiac tissues.

ABSTRACT:

Objectives

To establish a protocol to prepare and transplant clinical-grade human induced pluripotent stem cell (hiPSC)-derived cardiac tissues (HiCTs) and to evaluate the therapeutic potential in an animal myocardial infarction (MI) model.

Methods

We simultaneously differentiated clinical-grade hiPSCs into cardiovascular cell lineages with or without the administration of canonical Wnt inhibitors, generated 5- layer cell sheets with insertion of gelatin hydrogel microspheres (GHMs) (HiCTs), and transplanted them onto an athymic rat MI model. Cardiac function was evaluated by echocardiography and cardiac magnetic resonance imaging and compared with that in animals with sham and transplantation of 5-layer cell sheets without GHMs. Graft survival, ventricular remodeling, and n

SUBMITTER: Osada H 

PROVIDER: S-EPMC9390608 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature