Use of Magnetic Modulation of Nitrogen-Vacancy Center Fluorescence in Nanodiamonds for Quantitative Analysis of Nanoparticles in Organisms.

ABSTRACT: The fluorescence intensity emitted by nitrogen-vacancy (NV) centers in diamond nanoparticles can be readily modulated by the application of a magnetic field using a small electromagnet. By acquiring interleaved images acquired in the presence and absence of the magnetic field and performing digital subtraction, the fluorescence intensity of the NV nanodiamond can be isolated from scattering and autofluorescence even when these backgrounds are changing monotonically during the experiments. This approach has the potential to enable the robust identification of nanodiamonds in organisms and other complex environments. Yet, the practical application of magnetic modulation imaging to realistic systems requires the use of quantitative analysis methods based on signal-to-noise considerations. Her

SUBMITTER: Jones ZR 

PROVIDER: S-EPMC9390786 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature