Functional optimization of electric cell-substrate impedance sensing (ECIS) using human corneal epithelial cells.

ABSTRACT: An intact epithelium is key to maintaining corneal integrity and barrier function which can lead to impaired ocular defense and sight-threatening opacity when compromised. Electrical cell-substrate impedance sensing or ECIS is a non-invasive method to measure real-time cellular behaviors including barrier function and cell migration. The current study uses ECIS technology to assess and optimize human telomerase-immortalized corneal epithelial cells to generate quantifiable measurements that accurately reflect changes in cell behavior in vitro. Five cell densities were assessed in two different media to determine the optimal conditions for monitoring of cellular behavior over time. Parameters of evaluation included: overall impedance (Z), barrier resistance (R), cell capacitance (C), and ma

SUBMITTER: Ebrahim AS 

PROVIDER: S-EPMC9391335 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature