Cross-platform validation of a mouse blood gene signature for quantitative reconstruction of radiation dose.

ABSTRACT: In the search for biological markers after a large-scale exposure of the human population to radiation, gene expression is a sensitive endpoint easily translatable to in-field high throughput applications. Primarily, the ex-vivo irradiated healthy human blood model has been used to generate available gene expression datasets. This model has limitations i.e., lack of signaling from other irradiated tissues and deterioration of blood cells cultures over time. In vivo models are needed; therefore, we present our novel approach to define a gene signature in mouse blood cells that quantitatively correlates with radiation dose (at 1 Gy/min). Starting with available microarray datasets, we selected 30 radiation-responsive genes and performed cross-validation/training-testing data splits to downse

SUBMITTER: Ghandhi SA 

PROVIDER: S-EPMC9391341 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature