Scope and limitations of a multiplex conventional PCR for the diagnosis of S. stercoralis and hookworms.

ABSTRACT:

Objectives

Describe the diagnostic characteristics of a conventional multiplex PCR for the diagnosis of S. stercoralis, N. americanus and Ancylostomas spp.

Methods

Fecal samples were collected from a cross-sectional study in Orán department, Salta province, Argentina. The stool samples were analyzed using concentration-sedimentation, Harada Mori, McMaster, and Baermann techniques. DNA was extracted from 50 mg fecal sample using the FastPrep® Spin Kit for Soil. Three pairs of primers were used for the amplification of three products of 101, 330, and 577 base pairs (bp) for S. stercoralis, N. americanus and Ancylostoma spp, respectively. The sensitivity and analytical specificity of multiplex PCR were evaluated, as well as the sensitivity and diagnostic specificity, using a co

SUBMITTER: Fleitas PE 

PROVIDER: S-EPMC9392200 | biostudies-literature | 2021 Nov-Dec

REPOSITORIES: biostudies-literature