PDS5A and PDS5B differentially affect gene expression without altering cohesin localization across the genome.

ABSTRACT:

Background

Cohesin is an important structural regulator of the genome, regulating both three-dimensional genome organization and gene expression. The core cohesin trimer interacts with various HEAT repeat accessory subunits, yielding cohesin complexes of distinct compositions and potentially distinct functions. The roles of the two mutually exclusive HEAT repeat subunits PDS5A and PDS5B are not well understood.

Results

Here, we determine that PDS5A and PDS5B have highly similar localization patterns across the mouse embryonic stem cell (mESC) genome and they show a strong overlap with other cohesin HEAT repeat accessory subunits, STAG1 and STAG2. Using CRISPR/Cas9 genome editing to generate individual stable knockout lines for PDS5A and PDS5B, we find that loss of one PDS5 s

SUBMITTER: Arruda NL 

PROVIDER: S-EPMC9392266 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature