IPSCs derived from insulin resistant offspring of type 2 diabetic patients show increased oxidative stress and lactate secretion.

ABSTRACT:

Background

The genetic factors associated with insulin resistance (IR) are not well understood. Clinical studies on first-degree relatives of type 2 diabetic (T2D) patients, which have the highest genetic predisposition to T2D, have given insights into the role of IR in T2D pathogenesis. Induced pluripotent stem cells (iPSCs) are excellent tools for disease modeling as they can retain the genetic imprint of the disease. Therefore, in this study, we aimed to investigate the genetic perturbations associated with insulin resistance (IR) in the offspring of T2D parents using patient-specific iPSCs.

Methods

We generated iPSCs from IR individuals (IR-iPSCs) that were offspring of T2D parents as well as from insulin-sensitive (IS-iPSCs) individuals. We then performed transcriptomic

SUBMITTER: Memon B 

PROVIDER: S-EPMC9392338 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature