Nano-organization of spontaneous GABAergic transmission directs its autonomous function in neuronal signaling.

ABSTRACT: Earlier studies delineated the precise arrangement of proteins that drive neurotransmitter release and postsynaptic signaling at excitatory synapses. However, spatial organization of neurotransmission at inhibitory synapses remains unclear. Here, we took advantage of the molecularly specific interaction of antimalarial artemisinins and the inhibitory synapse scaffold protein, gephyrin, to probe the functional organization of gamma-aminobutyric acid A receptor (GABA_AR)-mediated neurotransmission in central synapses. Short-term application of artemisinins severely contracts the size and density of gephyrin and GABAaR γ2 subunit clusters. This size contraction elicits a neuronal activity-independent increase in Bdnf expression due to a specific reduction in GABAergic spontaneous, b

SUBMITTER: Guzikowski NJ 

PROVIDER: S-EPMC9392417 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature