Dataset Information

Exploring the anticancer and antioxidant properties of Vicia faba L. pods extracts, a promising source of nutraceuticals.

ABSTRACT:

Background

Pulse crops are considered the major sources of proteins, dietary fiber, micronutrients, and bioactive phytochemicals. Among the numerous pulse crops, broad beans (Vicia faba L.) have received particular attention due to their nutraceutical, functional and economic importance. Our attention was mainly focused on the broad bean pods (VFs), which are the primary by-product of the domestic and industrial processing of broad beans and an attractive source of valuable ingredients.

Methods

In order to investigate the VFs properties, the flours from broad beans of three different harvest periods were extracted with acetone, methanol and 70% aqueous ethanol and the dried extracts were analyzed, qualitatively and quantitatively, and tested for their antioxidant through DPPH and ABTS assay and anticancer activities using the MTT assay and immunofluorescence analysis.

Results

The VF extracts demonstrated a good in vitro radical scavenging activity from the first stage of collection of all the V. faba L. extracts. Additionally, the extracts were tested for their cytotoxicity against a panel of cancer and normal cells and the outcomes indicated the ethanol extract as the most active against the melanoma cell line Sk-Mel-28, without affecting the viability of the normal cells. Finally, we found out that the ethanol extract interfered with the microtubules organization, leading to the cancer cells death by apoptosis.

SUBMITTER: Ceramella J

PROVIDER: S-EPMC9392456 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature

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Publications

Exploring the anticancer and antioxidant properties of <i>Vicia faba</i> L. pods extracts, a promising source of nutraceuticals.

Ceramella Jessica J La Torre Chiara C De Luca Michele M Iacopetta Domenico D Fazio Alessia A Catalano Alessia A Ragno Gaetano G Longo Pasquale P Sinicropi Maria Stefania MS Rosano Camillo C

PeerJ 20220817

<h4>Background</h4>Pulse crops are considered the major sources of proteins, dietary fiber, micronutrients, and bioactive phytochemicals. Among the numerous pulse crops, broad beans (<i>Vicia faba</i> L.) have received particular attention due to their nutraceutical, functional and economic importance. Our attention was mainly focused on the broad bean pods (VFs), which are the primary by-product of the domestic and industrial processing of broad beans and an attractive source of valuable ingred ...[more]

PMID: 35996664

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