Simultaneous, multiplex quantification of protease activities using a gold microelectrode array.

ABSTRACT: Proteases are a large family of enzymes involved in many important biological processes. Quantitative detection of the activity profile of specific target proteases is in high demand for the diagnosis and monitoring of diseases such as cancers. This study demonstrates the fabrication and characterization of an individually addressable 3 × 3 Au microelectrode array for rapid, multiplex detection of cathepsin B activity based on a simple electrochemical method. The nine individual microelectrodes in the array show highly consistent cyclic voltammetric signals in Au surface cleaning experiments and detecting benchmark redox species in solution. The individual Au microelectrodes are further selectively functionalized with specific ferrocene-labeled peptide molecules which serve as the cognate

SUBMITTER: Anderson MJ 

PROVIDER: S-EPMC9392473 | biostudies-literature | 2020 Oct

REPOSITORIES: biostudies-literature