Dataset Information

Complete genome sequence of a multiple-stress-tolerant bacterium Halomonas piezotolerans NBT06E8^T isolated from a deep-sea sediment sample of the New Britain Trench.

ABSTRACT: Halomonas piezotolerans NBT06E8^T is a Gram-stain-negative, moderately halophilic, piezotolerant, H₂O₂ and heavy metal-resistant bacterium, isolated from a deep-sea sediment sample collected from the New Britain Trench at depth of 8900 m. Growth of the strain was observed at 4-45 °C (optimum 30 °C), at pH 5-11 (optimum 8-9) and in 0.5-21% (w/v) NaCl (optimum 3-7%). The optimum pressure for growth was 0.1-30 MPa (megapascal) with tolerance up to 60 MPa. Under optimum growth conditions, the strain could tolerant 15 mM H₂O₂. Here, we report the complete genome of H. piezotolerans NBT06E8^T, which consists of 3,945,801 bp (G + C content of 57.93%) with a single chromosome, 3509 protein-coding genes, 60 tRNAs and 6 rRNA operons. Genomic analysis revealed the capability of utilizing various carbon and nitrogen sources, the presence of multiple toxin-antitoxin systems and strain-specific type VI secretion system benefitting its adaptation to the oligotrophic hadal environments. Multiple respiratory chain components, especially the strain-specific anaerobic enzymes, could allow its survival in both surficial and buried sediments with variable oxygen concentrations. Gene function and metabolic pathway analysis showed that strain NBT06E8^T encodes a series of genes related to high hydrostatic pressure tolerance, antioxidative stress and heavy metal resistance, which could also contribute to its deep-sea adaptation strategies. The complete genome sequence of H. piezotolerans NBT06E8^T provides further insights into the stress adaptation strategies of deep-sea bacteria and potential biotechnological application of Halomonas species.

Supplementary information

The online version contains supplementary material available at 10.1007/s13205-022-03283-3.

SUBMITTER: Wang J

PROVIDER: S-EPMC9392676 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature

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Complete genome sequence of a multiple-stress-tolerant bacterium Halomonas piezotolerans NBT06E8T isolated from a deep-sea sediment sample of the New Britain Trench.

Wang Jiahua J Xie Zhe Z Liu Ying Y Yan Fangfang F Cao Junwei J Liu Rulong R Wang Li L Wei Yuli Y Fang Jiasong J

3 Biotech 20220820 9

Halomonas piezotolerans NBT06E8T is a Gram-stain-negative, moderately halophilic, piezotolerant, H2O2 and heavy metal-resistant bacterium, isolated from a deep-sea sediment sample collected from the New Britain Trench at depth of 8900 m. Growth of the strain was observed at 4-45 °C (optimum 30 °C), at pH 5-11 (optimum 8-9) and in 0.5-21% (w/v) NaCl (optimum 3-7%). The optimum pressure for growth was 0.1-30 MPa (megapascal) with tolerance up to 60 MPa. Unde ...[more]

PMID: 35999911

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Dataset Information

Complete genome sequence of a multiple-stress-tolerant bacterium Halomonas piezotolerans NBT06E8^T isolated from a deep-sea sediment sample of the New Britain Trench.

Supplementary information

Publications

Complete genome sequence of a multiple-stress-tolerant bacterium <i>Halomonas piezotolerans</i> NBT06E8<sup>T</sup> isolated from a deep-sea sediment sample of the New Britain Trench.

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