Gemin5-dependent RNA association with polysomes enables selective translation of ribosomal and histone mRNAs.

ABSTRACT: Selective translation allows to orchestrate the expression of specific proteins in response to different signals through the concerted action of cis-acting elements and RNA-binding proteins (RBPs). Gemin5 is a ubiquitous RBP involved in snRNP assembly. In addition, Gemin5 regulates translation of different mRNAs through apparently opposite mechanisms of action. Here, we investigated the differential function of Gemin5 in translation by identifying at a genome-wide scale the mRNAs associated with polysomes. Among the mRNAs showing Gemin5-dependent enrichment in polysomal fractions, we identified a selective enhancement of specific transcripts. Comparison of the targets previously identified by CLIP methodologies with the polysome-associated transcripts revealed that only a fraction of the t

SUBMITTER: Embarc-Buh A 

PROVIDER: S-EPMC9392717 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature