AFM-based nanoindentation indicates an impaired cortical stiffness in the AAV-PCSK9^DY atherosclerosis mouse model.

ABSTRACT: Investigating atherosclerosis and endothelial dysfunction has mainly become established in genetically modified ApoE^-/- or LDL-R^-/- mice transgenic models. A new AAV-PCSK9DY^DY mouse model with no genetic modification has now been reported as an alternative atherosclerosis model. Here, we aimed to employ this AAV-PCSK9^DY mouse model to quantify the mechanical stiffness of the endothelial surface, an accepted hallmark for endothelial dysfunction and forerunner for atherosclerosis. Ten-week-old male C57BL/6 N mice were injected with AAV-PCSK9^DY (0.5, 1 or 5 × 10¹¹ VG) or saline as controls and fed with Western diet (1.25% cholesterol) for 3 months. Total cholesterol (TC) and triglycerides (TG) were measured after 6 and 12 weeks

SUBMITTER: Achner L 

PROVIDER: S-EPMC9393126 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature