A new technique for stain-marking of seeds with safranine to track seed dispersal and seed bank dynamics.

ABSTRACT: Accurate tracking of seed dispersal is critical for understanding gene flow and seed bank dynamics, and for predicting population distributions and spread. Available seed-tracking techniques are limited due to environmental and safety issues or requirements for expensive and specialized equipment. Furthermore, few techniques can be applied to studies of water-dispersed seeds. Here we introduce a new seed-tracking method using safranine to stain seeds/fruits by immersing in (ex situ) or spraying with (in situ) staining solution. The hue difference value between pre- and post-stained seeds/fruits was compared using the HSV color model to assess the effect of staining. A total of 181 kinds of seeds/fruits out of 233 tested species of farmland weeds, invasive alien herbaceous pla

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC9393530 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature