Molecular characterization of NDM-1-producing carbapenem-resistant E. cloacae complex from a tertiary hospital in Chongqing, China.

ABSTRACT:

Background

The aim of this study was to clarify the molecular characterization of NDM-1-producing carbapenem-resistant Enterobacter cloacae complex (CREL) at a teaching hospital in Chongqing, China.

Methods

Antimicrobial susceptibility and resistance genes were analyzed. Epidemiological relationship was analyzed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). Conjugation experiments were performed to determine the transferability of plasmids. Whole-genome sequencing (WGS) of strains was implemented, and the genetic environment of the bla _NDM-1- and mcr-9-carrying plasmids was analyzed.

Results

A total of 10 bla _NDM-1-positive CREL isolates were identified. All isolates harbored multipl

SUBMITTER: Hu K 

PROVIDER: S-EPMC9393607 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature