Hyperinsulinemia alters insulin receptor presentation and internalization in brain microvascular endothelial cells.

ABSTRACT: Insulin receptors are internalized by endothelial cells to facilitate their physiological processes; however, the impact of hyperinsulinemia in brain endothelial cells is not known. Thus, the aim of this study was to elucidate the impact hyperinsulinemia plays on insulin receptor internalization through changes in phosphorylation, as well as the potential impact of protein tyrosine phosphatase 1B (PTP1B). Hippocampal microvessels were isolated from high-fat diet fed mice and assessed for insulin signaling activation, a process known to be involved with receptor internalization. Surface insulin receptors in brain microvascular endothelial cells were labelled to assess the role hyperinsulinemia plays on receptor internalization in response to stimulation, with and without the PTP1B antagonis

SUBMITTER: Watson LS 

PROVIDER: S-EPMC9393688 | biostudies-literature | 2022 Jul-Aug

REPOSITORIES: biostudies-literature