An additional regulatory gene for actinorhodin production in Streptomyces lividans involves a LysR-type transcriptional regulator.

ABSTRACT: The sequence of a 4.8-kbp DNA fragment adjacent to the right-hand end of the actinorhodin biosynthetic (act) cluster downstream of actVB-orf6 from Streptomyces coelicolor A3(2) reveals six complete open reading frames, named orf7 to orf12. The deduced amino acid sequences from orf7, orf10, and orf11 show significant similarities with the following products in the databases: a putative protein from the S. coelicolor SCP3 plasmid, LysR-type transcriptional regulators, and proteins belonging to the family of short-chain dehydrogenases/reductases, respectively. The deduced product of orf8 reveals low similarities with several methyltransferases from different sources, while orf9 and orf12 products show no similarities with other known proteins. Disruptions of orf10 and orf11 genes in S. coelic

SUBMITTER: Martinez-Costa OH 

PROVIDER: S-EPMC93938 | biostudies-literature | 1999 Jul

REPOSITORIES: biostudies-literature