Treatment of wild-type mice with 2,3-butanediol, a urinary biomarker of Fmo5 ^-/- mice, decreases plasma cholesterol and epididymal fat deposition.

ABSTRACT: We previously showed that Fmo5 ^-/- mice exhibit a lean phenotype and slower metabolic ageing. Their characteristics include lower plasma glucose and cholesterol, greater glucose tolerance and insulin sensitivity, and a reduction in age-related weight gain and whole-body fat deposition. In this paper, nuclear magnetic resonance (NMR) spectroscopy-based metabolite analyses of the urine of Fmo5 ^-/- and wild-type mice identified two isomers of 2,3-butanediol as discriminating urinary biomarkers of Fmo5 ^-/- mice. Antibiotic-treatment of Fmo5 ^-/- mice increased plasma cholesterol concentration and substantially reduced urinary excretion of 2,3-butanediol isomers, indicating that the gut microbiome contributed t

SUBMITTER: Veeravalli S 

PROVIDER: S-EPMC9393927 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature