Production of extracellular amylase contributes to the colonization of Bacillus cereus 0-9 in wheat roots.

ABSTRACT:

Background

Bacteria usually secrete a variety of extracellular enzymes to degrade extracellular macromolecules to meet their nutritional needs and enhance their environmental adaptability. Bacillus cereus 0-9, a biocontrol bacterial strain isolated from wheat roots, has three genes annotated as encoding amylases in the genome, but their functions are unknown, and whether they are involved in the colonization process of the bacterium remains to be further studied.

Methods

Mutant gene strains and fluorescently tagged strains were constructed by homologous recombination, and amylase protein was expressed in the prokaryotic Escherichia coli BL21(DE3) expression system. The iodine staining method was used to measure the activity of amylase proteins. We further observed the coloni

SUBMITTER: Huang Q 

PROVIDER: S-EPMC9394064 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature