NK cell-mediated immunostimulatory effects of ethanol extract of Morinda citrifolia (noni) fruit.

ABSTRACT:

Background

Morinda citrifolia (Noni) is a plant that has long been used in various products such as foods and cosmetics. Although noni has been known to have immunostimulatory activity, detailed mechanism at the cellular level has not been fully elucidated yet. In this study, we focused on understanding as to how noni fruit can positively stimulate body's immune responses.

Methods

To do this, an ethanol extract of noni fruit (Mc-fEE) was prepared and administered for 30 days to male C57BL/6 mice for in vivo experiment. NK cell activity and cytokine production level from Mc-fEE-treated mice were analyzed by flowcytometry, real-time PCR, and ELISA. Mc-fEE-triggered molecular events were detected from RAW264.7 cells and splenocytes using Western blotting and real-time PCR analy

SUBMITTER: Kim H 

PROVIDER: S-EPMC9394078 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature