Amplicon Sequencing Reveals Novel Fungal Species Responsible for a Controversial Tea Disease.

ABSTRACT: Amplicon sequencing is a powerful tool for analyzing the fungal composition inside plants, whereas its application for the identification of etiology for plant diseases remains undetermined. Here, we utilize this strategy to clarify the etiology responsible for tea leaf brown-black spot disease (LBSD), a noticeable disease infecting tea plants etiology that remains controversial. Based on the ITS-based amplicon sequencing analysis, Didymella species were identified as separate from Pestalotiopsis spp. and Cercospora sp., which are concluded as the etiological agents. This was further confirmed by the fungal isolation and their specific pathogenicity on diverse tea varieties. Based on the morphologies and phylogenetic analysis constructed with multi-loci (ITS, LSU, t

SUBMITTER: He Y 

PROVIDER: S-EPMC9394346 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature