A PCR-RFLP Technique to Assess the Geographic Origin of Plasmodium falciparum Strains in Central America.

ABSTRACT: The elimination of malaria requires strengthening diagnosis and offering adequate and timely treatment. Imported cases of falciparum malaria represent a major challenge for pre-elimination areas, such as Central America, where chloroquine and primaquine continue to be used as first-line treatment. The pfs47 gene has been previously described as a precise molecular marker to track the geographic origin of the parasite. The aim of this study was to design a simple and low-cost technique using the polymorphic region of pfs47 to assess the geographic origin of P. falciparum strains. A PCR-RFLP technique was developed and evaluated using the MseI enzyme that proved capable of discriminating, with reasonable precision, the geographical origin of the parasites. This me

SUBMITTER: Fontecha G 

PROVIDER: S-EPMC9394469 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature