Mitochondrial Ca²⁺ Uptake Drives Endothelial Injury By Radiation Therapy.

ABSTRACT:

Background

Radiation therapy strongly increases the risk of atherosclerotic vascular disease, such as carotid stenosis. Radiation induces DNA damage, in particular in mitochondria, but the upstream and downstream signaling events are poorly understood. The objective of this study was to define such mechanisms.

Methods

Endothelial-specific MCU (mitochondrial Ca²⁺ uniporter) knockout and C57Bl6/J mice with or without a preinfusion of a mitoTEMPO (mitochondrial reactive oxygen species [ROS] scavenger) were exposed to a single dose of cranial irradiation. 24, and 240 hours postirradiation, vascular reactivity, endothelial function, and mitochondrial integrity were assessed ex vivo and in vitro.

Results

In cultured human endothelial cells, irradiation with 4 Gy

SUBMITTER: Ait-Aissa K 

PROVIDER: S-EPMC9394506 | biostudies-literature | 2022 Sep

REPOSITORIES: biostudies-literature