Induced variations of ethyl methane sulfonate mutagenized cowpea (Vigna unguiculata L. walp) plants.

ABSTRACT: Unique variants are desired in the development of genetically improved crops to meet farmer and market needs hence ethyl methane sulfonate (EMS) was used to induce genetic variability in cowpea (Vigna unguiculata cv. Asontem). The main objective of this research was to characterize induced variations in EMS chemically mutagenized population of cowpea (Vigna unguiculata L. Walp Var. Asontem) in the M₁ and M₂ generations. The optimum concentration (LD50) of EMS for generating the mutagenized population was determined by treating seeds with different concentrations of EMS (0.0, 0.2, 0.4, 0.6, and 0.8% v/v) and observing the germination count after 5 days of planting the seeds in Petri dishes. Three thousand cowpea seeds were treated with the 0.4% EMS

SUBMITTER: Opoku Gyamfi M 

PROVIDER: S-EPMC9394701 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature