SilicoDArT and SNP markers for genetic diversity and population structure analysis of Trema orientalis; a fodder species.

ABSTRACT: Establishing the genetic diversity and population structure of a species can guide the selection of appropriate conservation and sustainable utilization strategies. Next-generation sequencing (NGS) approaches are increasingly being used to generate multi-locus data for genetic structure determination. This study presents the genetic structure of a fodder species -Trema orientalis based on two genome-wide high-throughput diversity array technology (DArT) markers; silicoDArT and single nucleotide polymorphisms (SNPs). Genotyping of 119 individuals generated 40,650 silicoDArT and 4767 SNP markers. Both marker types had a high average scoring reproducibility (>99%). Genetic relationships explored by principal coordinates analysis (PCoA) showed that the first principal coordinate axis explained

SUBMITTER: Nantongo JS 

PROVIDER: S-EPMC9394841 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature