First report of Leishmania RNA virus 1 in Leishmania (Viannia) braziliensis clinical isolates from Rio de Janeiro State - Brazil.

ABSTRACT:

Background

Leishmania parasites carry a double-stranded RNA virus (Leishmania RNA virus - LRV) that has been divided in LRV1 and LRV2.

Objectives

Leishmania (Viannia) braziliensis clinical isolates were assessed in order to determine LRV presence.

Methods

Two-round polymerase chain reaction (PCR and nested PCR) was performed to detect LRV1 or LRV2 in L. (V.) braziliensis clinical isolates (n = 12).

Findings

LRV1 was detected in three clinical isolates which was phylogenetically related to other sequences reported from other American tegumentary leishmaniasis (ATL) endemic areas of Brazil. Patients infected with L. (V.) braziliensis LRV-negative showed only cutaneous lesions while LRV-positive reported different manifestations.

Main conclusion

Data presen

SUBMITTER: Zabala-Penafiel A 

PROVIDER: S-EPMC9395166 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature