Transgenic quails reveal dynamic TCF/β-catenin signaling during avian embryonic development.

ABSTRACT: The Wnt/β-catenin signaling pathway is highly conserved throughout evolution, playing crucial roles in several developmental and pathological processes. Wnt ligands can act at a considerable distance from their sources and it is therefore necessary to examine not only the Wnt-producing but also the Wnt-receiving cells and tissues to fully appreciate the many functions of this pathway. To monitor Wnt activity, multiple tools have been designed which consist of multimerized Wnt signaling response elements (TCF/LEF binding sites) driving the expression of fluorescent reporter proteins (e.g. GFP, RFP) or of LacZ. The high stability of those reporters leads to a considerable accumulation in cells activating the pathway, thereby making them easily detectable. However, this makes them unsuitable

SUBMITTER: Barzilai-Tutsch H 

PROVIDER: S-EPMC9395189 | biostudies-literature | 2022 Jul

REPOSITORIES: biostudies-literature