Assessment of creatinine concentration in whole blood spheroids using paper spray ionization-tandem mass spectrometry.

ABSTRACT: Accurate quantification of blood creatinine is important to estimate the glomerular filtration rate. Existing techniques using liquid chromatography tandem mass spectrometry (LC-MS/MS) have a high accuracy and eliminate most interferences encountered in routine enzymatic and Jaffé methods. However, they require laborious and time-consuming sample treatment and data acquisition. The aim of this study is to develop a fast and simple method to enable a direct analysis of whole blood creatinine with performance measures that are comparable to conventional LC-MS/MS. 5μL whole blood is formed as a three-dimensional spheroid on hydrophobic silanized paper substrates which then undergoes paper-spray ionization-tandem mass spectrometry (PSI-MS/MS). The method is validated using real human samples a

SUBMITTER: Sham TT 

PROVIDER: S-EPMC9395369 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature