RSPO3 is a novel contraction-inducible factor identified in an "in vitro exercise model" using primary human myotubes.

ABSTRACT: The physiological significance of skeletal muscle as a secretory organ is now well known but we can only speculate as to the existence of as-yet-unidentified myokines, especially those upregulated in response to muscle contractile activity. We first attempted to establish an "insert-chamber based in vitro exercise model" allowing the miniature but high cell-density culture state enabling highly developed contractile human myotubes to be readily obtained by applying electric pulse stimulation (EPS). By employing this in vitro exercise model, we identified R-spondin 3 (RSPO3) as a novel contraction-inducible myokine produced by cultured human myotubes. Contraction-dependent muscular RSPO3 mRNA upregulation was confirmed in skeletal muscles of mice subjected to sciatic nerve mediated in situ

SUBMITTER: Takahashi T 

PROVIDER: S-EPMC9395423 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature