Xenogeneic Humoral Immune Responses to Human Mesenchymal Stem Cells in Mice.

ABSTRACT:

Background and objectives

Many preclinical studies have been conducted using animal disease models to determine the effectiveness of human mesenchymal stem cells (hMSCs) for treating immune and inflammatory diseases based on the belief that hMSCs are not immunogenic across species. However, several researchers have suggested xenogeneic immune responses to hMSCs in animals, still without detailed features. This study aimed to investigate a xenogeneic humoral immune response to hMSCs in mice in detail.

Methods and results

Balb/c mice were intraperitoneally injected with adipose tissue-derived or Wharton's jelly-derived hMSCs. Sera from these mice were titrated for each isotype. To confirm specificity of the antibodies, hMSCs were stained with the sera and subjected to a flow c

SUBMITTER: Hong JM 

PROVIDER: S-EPMC9396016 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature