Limited Evidence for Protein Products of Noncoding Transcripts in the HEK293T Cellular Cytosol.

ABSTRACT: Ribosome profiling has revealed translation outside canonical coding sequences, including translation of short upstream ORFs, long noncoding RNAs, overlapping ORFs, ORFs in UTRs, or ORFs in alternative reading frames. Studies combining mass spectrometry, ribosome profiling, and CRISPR-based screens showed that hundreds of ORFs derived from noncoding transcripts produce (micro)proteins, whereas other studies failed to find evidence for such types of noncanonical translation products. Here, we attempted to discover translation products from noncoding regions by strongly reducing the complexity of the sample prior to mass spectrometric analysis. We used an extended database as the search space and applied stringent filtering of the identified peptides to find evidence for novel translation ev

SUBMITTER: Bogaert A 

PROVIDER: S-EPMC9396073 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature