Optimization of quantitative reverse transcription PCR method for analysis of weakly expressed genes in crops based on rapeseed.

ABSTRACT: Rapeseed (Brassica napus) is an allopolyploid hybrid (AACC genome) of turnip rape (B. rapa, genome: AA) and vegetable cabbage (B. oleraceae, genome: CC). Rapeseed oil is one of the main vegetable oils used worldwide for food and other technical purposes. Therefore, breeding companies worldwide are interested in developing rapeseed varieties with high yields and increased adaptation to harsh climatic conditions such as heat and prolonged drought. One approach to studying the mechanism of the epigenetically regulated stress response is to analyze the transcriptional changes it causes. In addition, comparing the expression of certain genes between stress- and non-stress-tolerant varieties will help guide breeding in the desired direction. Quantitative reverse transcriptio

SUBMITTER: Moebes M 

PROVIDER: S-EPMC9396215 | biostudies-literature | 2022

REPOSITORIES: biostudies-literature