Cre-recombinase systems for induction of neuron-specific knockout models: a guide for biomedical researchers.

ABSTRACT: Gene deletion has been a valuable tool for unraveling the mysteries of molecular biology. Early approaches included gene trapping and gene targetting to disrupt or delete a gene randomly or at a specific location, respectively. Using these technologies in mouse embryos led to the generation of mouse knockout models and many scientific discoveries. The efficacy and specificity of these approaches have significantly increased with the advent of new technology such as clustered regularly interspaced short palindromic repeats for targetted gene deletion. However, several limitations including unwanted off-target gene deletion have hindered their widespread use in the field. Cre-recombinase technology has provided additional capacity for cell-specific gene deletion. In this review, we provide a

SUBMITTER: Shcholok T 

PROVIDER: S-EPMC9396489 | biostudies-literature | 2023 Feb

REPOSITORIES: biostudies-literature