Simple, Rapid Chemical Labeling and Screening of Antibodies with Luminescent Peptides.

ABSTRACT: Sensitive and selective detection assays are essential for the accurate measurement of analytes in both clinical and research laboratories. Immunoassays that rely on nonoverlapping antibodies directed against the same target analyte (e.g., sandwich enzyme-linked immunosorbent assays (ELISAs)) are commonly used molecular detection technologies. Use of split enzyme reporters has simplified the workflow for these traditionally complex assays. However, identifying functional antibody pairs for a given target analyte can be cumbersome, as it generally involves generating and screening panels of antibodies conjugated to reporters. Accordingly, we sought a faster and easier reporter conjugation strategy to streamline antibody screening. We describe here the development of such a method that is ba

SUBMITTER: Kincaid VA 

PROVIDER: S-EPMC9396617 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature