Characterizing a New Fluorescent Protein for a Low Limit of Detection Sensing in the Cell-Free System.

ABSTRACT: Cell-free protein synthesis-based biosensors have been developed as highly accurate, low-cost biosensors. However, since most biomarkers exist at low concentrations in various types of biopsies, the biosensor's dynamic range must be increased in the system to achieve low limits of detection necessary while deciphering from higher background signals. Many attempts to increase the dynamic range have relied on amplifying the input signal from the analyte, which can lead to complications of false positives. In this study, we aimed to increase the protein synthesis capability of the cell-free protein synthesis system and the output signal of the reporter protein to achieve a lower limit of detection. We utilized a new fluorescent protein, mNeonGreen, which produces a higher output than those co

SUBMITTER: Copeland CE 

PROVIDER: S-EPMC9396652 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature