Proteolytically Activated CRAC Effectors through Designed Intramolecular Inhibition.

ABSTRACT: Highly regulated intracellular calcium entry affects numerous cellular physiological events. External regulation of intracellular calcium signaling presents a great opportunity for the artificial regulation of cellular activity. Calcium entry can be mediated by STIM proteins interacting with Orai calcium channels; therefore, the STIM1-Orai1 pair has become a tool for artificially modulating calcium entry. We report on an innovative genetically engineered protease-activated Orai activator called PACE. CAD self-dimerization and activation were inhibited with a coiled-coil forming peptide pair linked to CAD via a protease cleavage site. PACE generated sustained calcium entry after its activation with a reconstituted split protease. We also generated PACE, whose transcriptional activation of N

SUBMITTER: Jazbec V 

PROVIDER: S-EPMC9396659 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature