Building the Plant SynBio Toolbox through Combinatorial Analysis of DNA Regulatory Elements.

ABSTRACT: While the installation of complex genetic circuits in microorganisms is relatively routine, the synthetic biology toolbox is severely limited in plants. Of particular concern is the absence of combinatorial analysis of regulatory elements, the long design-build-test cycles associated with transgenic plant analysis, and a lack of naming standardization for cloning parts. Here, we use previously described plant regulatory elements to design, build, and test 91 transgene cassettes for relative expression strength. Constructs were transiently transfected into Nicotiana benthamiana leaves and expression of a fluorescent reporter was measured from plant canopies, leaves, and protoplasts isolated from transfected plants. As anticipated, a dynamic level of expression was achieved from the l

SUBMITTER: Pfotenhauer AC 

PROVIDER: S-EPMC9396662 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature