BMAL1 plays a critical role in the protection against cardiac hypertrophy through autophagy in vitro.

ABSTRACT:

Background

Heart disease could result from a malfunction in the core clock gene BMAL1, according to studies conducted on animals and humans in vitro and in vivo. However, in pathological conditions, the role of BMAL1 was not clear. In the present study, we identified a potential link between BMAL1 and cardiac hypertrophy.

Methods

Primary cultured neonatal rat cardiomyocytes were stimulated by Ang II. Cardiomyocytes immunofluorescence analysis was performed to observe the cell size. RT-PCR and Western blot were used to find out the gene and protein expression. Cell apoptosis was measured by TUNEL staining. The Elisa assay was performed which determine the release of cytokines led to the activation of cardiac fibro-blasts in cell-free supernatants. Furthermore, gain- and loss-

SUBMITTER: Yu L 

PROVIDER: S-EPMC9396899 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature