Second-Generation Escherichia coli SuptoxR Strains for High-Level Recombinant Membrane Protein Production.

ABSTRACT: Escherichia coli is one of the most widely utilized hosts for recombinant protein production, including that of membrane proteins (MPs). We have recently engineered a specialized E. coli strain for enhanced recombinant MP production, termed SuptoxR. By appropriately co-expressing the effector gene rraA, SuptoxR can suppress the high toxicity, which is frequently observed during the MP-overexpression process, and, at the same time, enhance significantly the cellular accumulation of membrane-incorporated and properly folded recombinant MP. The combination of these two beneficial effects results in dramatically enhanced volumetric yields for various prokaryotic and eukaryotic MPs. Here, we engineered second-generation SuptoxR strains with further improved properties, so t

SUBMITTER: Vasilopoulou E 

PROVIDER: S-EPMC9397408 | biostudies-literature | 2022 Aug

REPOSITORIES: biostudies-literature